European Ocean Biodiversity Information System
[ report an error in this record ]basket (0): add | show Print this page
CALUX measurements: statistical inferences for the dose–response curve
Elskens, M.; Baston, D.S.; Stumpf, C.; Haedrich, J.; Keupers, I.; Croes, K.; Denison, M.S.; Baeyens, W.; Goeyens, L. (2011). CALUX measurements: statistical inferences for the dose–response curve. Talanta 85(4): 1966-1973. http://dx.doi.org/10.1016/j.talanta.2011.07.014
In: Talanta. Elsevier: Amsterdam. ISSN 0039-9140; e-ISSN 1873-3573
Peer reviewed article  
Available in  Authors 
    Vlaams Instituut voor de Zee: Open Marine Archive 279669 [ download pdf ]
Author keywords
    Bioassay CALUX; Statistical inference; Dose–response curve; Box–Cox transformation
Authors  Top 
  • Elskens, M.
  • Baston, D.S.
  • Stumpf, C.
  • Haedrich, J.
  • Keupers, I.
  • Croes, K.
  • Denison, M.S.
  • Baeyens, W.
  • Goeyens, L.
Abstract
    Chemical Activated LUciferase gene eXpression [CALUX] is a reporter gene mammalian cell bioassay used for detection and semi-quantitative analyses of dioxin-like compounds. CALUX dose–response curves for 2,3,7,8-tetrachlorodibenzo-p-dioxin [TCDD] are typically smooth and sigmoidal when the dose is portrayed on a logarithmic scale. Non-linear regression models are used to calibrate the CALUX response versus TCDD standards and to convert the sample response into Bioanalytical EQuivalents (BEQs). Several complications may arise in terms of statistical inference, specifically and most important is the uncertainty assessment of the predicted BEQ. This paper presents the use of linear calibration functions based on Box–Cox transformations to overcome the issue of uncertainty assessment. Main issues being addressed are (i) confidence and prediction intervals for the CALUX response, (ii) confidence and prediction intervals for the predicted BEQ-value, and (iii) detection/estimation capabilities for the sigmoid and linearized models. Statistical comparisons between different calculation methods involving inverse prediction, effective concentration ratios (ECR20–50–80) and slope ratio were achieved with example datasets in order to provide guidance for optimizing BEQ determinations and expand assay performance with the recombinant mouse hepatoma CALUX cell line H1L6.1c3.
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors