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Mesure de l'activité enzymatique éthoxyrésorufine-O-dééthylase (ÉROD) chez les poissons marins
Burgeot, T.; Ménard, D. (2004). Mesure de l'activité enzymatique éthoxyrésorufine-O-dééthylase (ÉROD) chez les poissons marins. Méthodes d'analyse en milieu marin. IFREMER: Plouzané. ISBN 2-84433-136-X. 26 pp.
Part of: Méthodes d'analyse en milieu marin. Editions IFREMER: Plouzané. ISSN 1637-1844
Available in  Authors 
    Vlaams Instituut voor de Zee: Non-open access 350375 [ request ]
Keywords
    ANE, English Channel
    ANE, North Sea
    Aquatic organisms > Marine organisms > Fish > Marine fish
    Biomarkers
    Detoxification
    Enzymatic activity
    Secretory organs > Glands > Exocrine glands > Digestive system > Digestive glands > Liver
    Standardization > Calibration > Intercalibration
    English Channel [Marine Regions]; North Sea [Marine Regions]
    Marine/Coastal
Authors  Top 
  • Burgeot, T.
  • Ménard, D.
Abstract
    Pilot studies conducted in the English Channel and North Sea, along with international intercalibration exercises in the 1990s, have helped validate the method for measurement of ethoxyresorufin-O-deethylase (EROD) which is dependent on the CYP4501A system. These stages have provided the basis to develop a detoxification biomarker for use in monitoring applications. The fluorescence-based EROD enzyme microplate assay has the advantage of being a fast, inexpensive and easily implemented method. The method described is the outcome of ten years of experience supported by the national observation network RNO. Some data on marine finfish collected in their natural environment and subjected to exposure in the laboratory are used to present a range of variations of use in interpreting EROD enzyme activity on the Channel, North Sea and Mediterranean coasts.
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